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Raman Spectroscopic Imaging: Research Applications in Oncology and Drug Delivery
||Raman spectroscopy is routinely used for characterization and identification of inorganic materials.
||Wednesday, June 11, 2014 12:00 PM
Wednesday, June 11, 2014
||Medical Science Building - Rbhs
||185 South Orange Avenue
|City, State, Country:
||The NJMS Research Office and Core Facilities
||Talk, Lecture, Seminar
||Robert Donnelly, Ph.D.
||Raman spectroscopy is routinely used for characterization and identification of inorganic materials. However, recent advances integrating Raman spectroscopy, confocal microscopy and imaging software have allowed biological samples such as cells, tissues and biofilms to be analyzed and mapped at micron level resolution. Raman spectral peaks are derived from vibrational modes of existing chemical groups and thus require no exogenous label – one directly monitors existing molecular components. This seminar will cover the application of Raman microscopy to cancer cells of varying metastatic capabilities and to the cochleate lipid-based drug delivery system. Using this approach, we have found evidence of diminished nuclear lipid ordering in the nuclei of highly metastatic cancer cells, which may contribute to transcriptional dysregulation. We have also demonstrated a measurable spectral difference between Amphotericin B completely encapsulated in a cochleate and external to the cochleate, making possible the visualization of drug release from the lipid delivery vessel inside of the target cells.